Supplementary Figure 6: IL-1 increases IL-21-mediated tyrosine-phosphorylation of STAT3 in TH17 cells. | Nature Immunology

Supplementary Figure 6: IL-1 increases IL-21-mediated tyrosine-phosphorylation of STAT3 in TH17 cells.

From: IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell–iTreg cell balance

Supplementary Figure 6

(a) Naïve CD4+ T cells were cultured under Th17 polarizing conditions for 4 days, then restimulated with IL-21 alone, IL-1β alone, or both for the indicated time periods. Cell lysates were harvested, immunoblotted with antibody directed against phospho-tyrosine(705)-STAT3 (pY-STAT3; upper panel) or total STAT3 (lower panel). (b) Pooled data representing integrated band density values (IDVs) of pY-STAT3 normalized to total STAT3 from a. Data are representative of one of two independent experiments (a) or are pooled from two independent experiments representing integrated band density values (IDVs) of pY-STAT3 normalized to total STAT3 from a (means and s.e.m. in b). *p<0.05 (two-tailed unpaired T-test).

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