Supplementary Figure 6: Tet1-deficient HSCs display increased self-renewal in vivo with a deficiency in bone marrow mature B cells and expression of genes in histone cluster 1 in hematopoietic cell lineages.
From: TET1 is a tumor suppressor of hematopoietic malignancy
a) Frequency of CD45.2+ competitive donor cells in total LSKs, LT-HSCs and MPPs from the bone marrow of primary transplanted mice 20-weeks post-reconstitution (mean ± SEM, n = 6 mice per genotype); * P = <0.001, ** P = <0.0001) (unpaired t-test). b) Representative flow cytometry of CD45.2+ cells from the bone marrow of primary transplanted mice. Upper panel; HSC subsets of CD45.2+ LSK cells stained for LT-HSC, ST-HSC, MPP1 and MPP2 with CD150 and CD48 as previously described. Lower panel; CD45.2+ B220+ cells stained for B cell subsets with IgM and IgD. c) Sorting strategy for microarray and RNA-seq analysis of total LSK, LT-HSC and MPP populations. d) Heat map of Histone cluster 1 gene expression in hematopoietic stem, progenitor and mature cells.
