Supplementary Figure 1: Peripheral blood analysis and organ histology of sick Tet1-deficient mice.
From: TET1 is a tumor suppressor of hematopoietic malignancy
a) Hemavet quantification of whole blood cell numbers from Tet1+/+, Tet1+/– and Tet1–/– mice aged 18-24 months. WBC = white blood cells, RBC = red blood cells, NS = not significant, * P = <0.05. (unpaired t-test) Small horizontal lines indicate the mean; Tet1+/+ (n = 20), Tet1+/– (n = 20) and Tet1–/– (n = 32). b-c) Peripheral blood smears stained with Wright-Giemsa and d) flow cytometric analysis of peripheral blood from sick Tet1-deficient mice compared to age-matched Tet1+/+ controls. Data are representative of 3 independent experiments, n = 8-10 mice per genotype. e) Histological analysis of spleens from sick Tet1+/– and Tet1–/– mice compared to age-matched Tet1+/+ controls. Sections were stained with H&E and Ki67 as indicated. RP = Red pulp, F = Follicle. f) H&E staining of liver, lung and kidney sections from sick Tet1+/– showing diffuse lymphocytic infiltration compared to age-matched Tet1+/+ mice. g) H&E and Ki67 staining of hyperproliferative infiltrating cells in liver, lung and kidney of Tet1-deficient mice. Histology is representative of Tet1+/+, Tet1+/– and Tet1–/– mice (n = 6-8 mice per genotype). Scale bar = 100μm in all panels.
