Supplementary Figure 4: Purification and survival of eosinophils. | Nature Immunology

Supplementary Figure 4: Purification and survival of eosinophils.

From: Eosinophils orchestrate cancer rejection by normalizing tumor vessels and enhancing infiltration of CD8+ T cells

Supplementary Figure 4

a) Mice were injected i.p. with thioglycollate and peritoneal cells where harvested 3 days later. b) Eosinophils were purified from these peritoneal cells by MACS using beads coated with anti-PE antibodies and Siglec-F PE labeled antibodies. The purity obtained was above 80%. c) Enriched eosinophils were activated in vitro with complete RPMI medium containing IFNγ (15 ng/ml) and TNF (20 ng/ml) up to 16h. After activation the purity was 90%. d) Viability of eosinophil after in vitro culture. n=6. 1 of 3 independent experiments is shown.

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