Supplementary Figure 7: Changes in the tumor microenvironment after transfer of eosinophils and OT-I cells. | Nature Immunology

Supplementary Figure 7: Changes in the tumor microenvironment after transfer of eosinophils and OT-I cells.

From: Eosinophils orchestrate cancer rejection by normalizing tumor vessels and enhancing infiltration of CD8+ T cells

Supplementary Figure 7

a) Flow cytometric quantification of tumor infiltrating leukocyte subpopulations after transfer of activated eosinophils and OTI cells. The data shows significantly more T-cells infiltrating the tumor after cotransfer of activated eosinophils and OT-I as compamagenta to OTI cells alone. b) Representative confocal images of MO4 tumors 2 days after transfer of activated tumor-specific CD8+ T cells and activated eosinophils. Bar, 50µm. n=7 mice per group. c) Q-PCR analysis of cytokine and chemokine levels in the tumor microenvironment after transfer of activated eosinophils and OTI cells. d) Q-PCR analysis of angiogenesis factors in the tumor microenvironment after transfer of activated eosinophils and OTI cells. n=6 mice. Results are shown as mean ±SEM. 1 of 2 independent experiments is shown *=p<0.05; **=p<0.01; ***=p<0.001; NS=not significant (unpaired T-test).

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