Supplementary Figure 4: Brg1 binds at de novo enhancers in pro-B cells. | Nature Immunology

Supplementary Figure 4: Brg1 binds at de novo enhancers in pro-B cells.

From: The chromatin remodeler Brg1 activates enhancer repertoires to establish B cell identity and modulate cell growth

Supplementary Figure 4

(a) Clustering centered on Ikaros-bound sites and further analyzed in a window of ± 3 kb for Ikaros occupancy in Tcf3−/− (E2A-deficient) pre-pro-B and Rag1−/− pro-B cells as well as Brg1 occupancy in Rag1−/− pro-B cells. Ikaros peaks were comprised of Ikaros binding sites in pre-pro-B cells and high-affinity binding sites in pro-B cells. (b) Nucleosome depletion was assessed through MNase sequencing. MNase-Seq tag coverage is assessed in Tcf3−/− pre-pro-B and Rag1−/− pro-B cells. MNase reads are plotted as a function of genomic distance from Brg1 bound-sites (identified in pro-B cells). ChIP-Seq and MNase-Seq data derived from one independent experiment.

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