Supplementary Figure 5: Brg1 regulates the binding of transcription factors in the Igh locus. | Nature Immunology

Supplementary Figure 5: Brg1 regulates the binding of transcription factors in the Igh locus.

From: The chromatin remodeler Brg1 activates enhancer repertoires to establish B cell identity and modulate cell growth

Supplementary Figure 5

(a) Expression of pro-B lineage-specific transcription factors in Rag1−/− cells transduced respectively with a control retrovirus (sh-Ctrl) and a retrovirus expressing a shRNA directed against Smarca4 (sh-Smarca4) determined by RNA-Seq. Expression represented as mean normalized counts. Mean from 3 independent experiments. (b) Igh locus contraction upon depletion of Smarca4 expression. Orthogonal projections derived from 3D-FISH experiments are shown. BAC probe RP23-201H14 is rendered in green and BAC probe RP24-189H12 in red. DAPI staining is shown in blue. (c) RNA-Seq tag counts representing antisense transcription on the Igh locus in sh-Ctrl and sh-Smarca4 Rag1−/− pro-B cells as well as Smarca4+/+ and Smarca4Δ/Δ pro-B cells is depicted and are representative of at least two independent experiments. Right panel, antisense transcription associated with PAIR elements 4 and 6 is downregulated in absence of Brg1.

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