Supplementary Figure 4: Treg cell development is enhanced in the absence of existing thymic Treg cells. | Nature Immunology

Supplementary Figure 4: Treg cell development is enhanced in the absence of existing thymic Treg cells.

From: Thymic regulatory T cell niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition

Supplementary Figure 4

(a) Slices from WT or AND (Treg deficient) thymi, overlaid with 2D2 TCR transgenic thymocytes in the presence of 3.8μM MOG 35-55 peptide. Treg cell development was analyzed on day 3 by flow cytometry. (b) Number of 2D2 Treg cells recovered per thymic slice after 3d. *p<0.01 by student’s t-test, n=6 and 3 slices respectively, representative of 2 independent experiments. (c) Slices from WT or F5 TCR transgenic Rag2-/-(Treg deficient) thymi, overlaid with OT-II thymocytes in the presence of the indicated concentrations of OVA peptide. Treg cell development analyzed on day 3 by flow cytometry. (d) Number of OT-II Treg cells recovered per thymic slice after 3d. *p<0.01 and **p<0.001 one-way ANOVA using Tukey’s post test analysis. Data pooled from 3 independent experiments, n=9 slices each.

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