Supplementary Figure 2: Treg cell development in thymic slices recapitulates normal thymic development rather than in vitro–induced Treg cell development. | Nature Immunology

Supplementary Figure 2: Treg cell development in thymic slices recapitulates normal thymic development rather than in vitro–induced Treg cell development.

From: Thymic regulatory T cell niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition

Supplementary Figure 2

(a) OT-II thymocytes or mature OT-II T cells were added to thymic slices from wild type mice that were injected with OVA protein 1hr prior to sacrifice. TGF-β (5ng/ml) was added along with mature OT-II T cells in some samples as indicated. (b) Neuropilin-1 expression levels on gated OT-II Foxp3+ CD4SP from thymocytes, splenocytes, or Foxp3 negative thymic CD4SP. (c) OT-II thymocytes were depleted of CD4SP prior to introduction into thymic slices. Flow cytometric analysis of samples before and after depletion are shown. (d) OT-II CD4SP depleted thymocytes overlaid onto WT thymic slices along with 1μM OVA peptide. Flow cytometry of gated OT-II CD4SP after days 3 or 4 of culture are shown. Data representative of 2 (a,b) or 3 (c,d) independent experiments.

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