Supplementary Figure 8: Expression of the genes encoding p21 and p27 is upregulated by Nurr1 overexpression. | Nature Immunology

Supplementary Figure 8: Expression of the genes encoding p21 and p27 is upregulated by Nurr1 overexpression.

From: The transcription factor Foxm1 is essential for the quiescence and maintenance of hematopoietic stem cells

Supplementary Figure 8

(a) Doxycycline-induced expression of Flag-Nurr1 in BM cells from the chimeric mice. The Flag-Nurr1 expression in BM cells was determined by Western Blot. The Foxm1â–³/â–³ BM cells expressing pLVX-Tet-On and pLVX-tight-puro-Nurr1 or pLVX-tight-puro or Foxm1fl/fl BM cells expressing pLVX-Tet-On and pLVX-tight-puro from chimeric mice received two-week-induction of Doxycyline. (b) qRT-PCR analysis of p21, p27 and p16 expression in LSK cells. The LSK cells, isolated from Foxm1fl/fl Tie2-Cre or control Foxm1fl/fl mice, were infected with MSCV-puro-Nurr1 or control vector and cultured with Stemspan medium with cytokines. The cells were treated with Puromycin for 2 days before analysis. Gene expression was initially normalized to Actb expression. Values represent the fold changes in gene expression relative to that in Foxm1fl/fl LSK cells expressing control vector. *, P<0.05.

Back to article page