Supplementary Figure 3: IL-26 is mainly expressed by primary CD4+ TH17 cells and TH17 cell clones. | Nature Immunology

Supplementary Figure 3: IL-26 is mainly expressed by primary CD4+ TH17 cells and TH17 cell clones.

From: TH17 cells promote microbial killing and innate immune sensing of DNA via interleukin 26

Supplementary Figure 3

(a) Microarray gene-expression profile in isolated unstimulated or activated peripheral blood immune cell subsets. TH1, TH2 and TH17 cells were differentiated from naive T cells as described in the Online Methods. The results of the gene-expression profile of IL-26 are shown as the relative hybridization intensity level. Error bars represent the s.d. of duplicates. (b) Real-time PCR analysis of IL17A and IL26 mRNA expression in re-stimulated TH17 clones 1G3, 7H1 and 72G6, transfected with siRNA targeting IL-26 (siIL-26) or a control siRNA (siCtl). The feeder cell line LCL was used as a control (-). Error bars are the s.d. of triplicate wells. Data are representative of two independent experiments.

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