Supplementary Figure 4: Antimicrobial activity and interferon-inducing capacity of recombinant and natural TH17 cell–derived IL-26.
From: TH17 cells promote microbial killing and innate immune sensing of DNA via interleukin 26
(a–c) Growth of P. aeruginosa treated with (a) increasing concentrations of rhIL-26, (b) supernatants of TH17 cell clones 1G3, 7H1, 72G6 or (c) supernatants of TH17 cell clones transfected with siRNA targeting IL-26 (siIL-26) or control siRNA (siCtl). Data are representative of two independent experiments. Data are means of duplicate wells. (d) IFN-α produced by pDCs stimulated overnight with exogenous human DNA in the presence of increasing concentrations of rhIL-26, IL-26–containing TH17 cell supernatants, or IL-26–containing TH17 cell supernatants treated with neutralizing anti–IL-26. Error bars represent the standard deviation of duplicate wells. Data are representative of two independent experiments.
