Supplementary Figure 7: IL-26–human DNA complex formation can be inhibited by anionic polymers and anti–IL-26.
From: TH17 cells promote microbial killing and innate immune sensing of DNA via interleukin 26
(a,b) Fluorimetric quantification of DNA staining by picogreen dye upon mixing of human DNA with IL-26 in the presence of (a) increasing concentrations of the anionic polymer heparin or (b) neutralizing anti–IL-26 or anti–IL-17. (c) IFN-α produced by pDCs stimulated overnight with IL-26–human DNA complexes in the presence of neutralizing anti–IL-26 or isotype control antibodies (ctl IgG). Error bars represent the s.d. of triplicate wells. Data are representative of three independent experiments. Statistical analysis was done using unpaired two-tailed Student’s t-test; *P < 0.001.
