Supplementary Figure 2: Antimicrobial activity of rhIL-26.
From: TH17 cells promote microbial killing and innate immune sensing of DNA via interleukin 26
(a) Growth of the virulent strains E. coli J5 O111:B4, E. coli O111:K58:H2, E. coli O18:K1:H7 and P. aeruginosa PA14 in culture with increasing concentrations of rhIL-26. (b) Flow cytometry analysis of K. pneumoniae O1:K2 (top) and P. aeruginosa PA14 (bottom) treated with increasing concentrations of rhIL-26 for 1 h and stained with SYTO 13 Green (permeant DNA dye) and SYTOX Orange (impermeant DNA dye). (c) Fluorescence microscopy imaging of K. pneumoniae O1:K2 in culture medium or treated for 1 h with 10 µM IL-26, and stained with SYTO 13 Green (permeant DNA dye) and SYTOX Orange (impermeant DNA dye). Left images show an overview of the bacteria; middle and right images show bacteria at higher magnification. (d) Growth of S. aureus ATCC 6538, E. coli O1:K1:H7 and P. aeruginosa ATCC 27853 in culture with increasing concentrations of rhIL-26 or LL-37. (e) Gorwth of E. coli O111:K58:H2, K. pneumoniae O1:K2 and P. aeruginosa PA14 in culture with 10 µM of IL-26, LL-37 or hBD3. a–e, Data are representative of three independent experiments. e, Error bars represent the s.d. of triplicates.
