Supplementary Figure 3: CD4+ Treg cells continue to produce IL-10 during the resolution phase of infection. | Nature Immunology

Supplementary Figure 3: CD4+ Treg cells continue to produce IL-10 during the resolution phase of infection.

From: Production of IL-10 by CD4+ regulatory T cells during the resolution of infection promotes the maturation of memory CD8+ T cells

Supplementary Figure 3

(a) Analysis of the GP33+ T cell response 60 days post acute LCMV infection in Il10f/f, Il10f/fCd4-Cre, Il10f/fLyz2-Cre, and Il10f/fCd11c-Cre mice. Percentages and numbers of GP33+ T cells were determined. (b) Analysis of IL-10 production by T cells following acute LCMV infection in IL-10 reporter (10BiT Thy1.1 mice). Percentages and numbers of IL-10-Thy1.1 reporter-positive cells at multiple time points post LCMV infection are shown. (c) Intravenously (i.v.) administered anti-CD4 antibody was used to distinguish circulating (red pulp localized) versus resident (white pulp localized) CD4+ T cells. (d) Analysis of the GP33+ T cell response 60 days post acute LCMV Armstrong infection. Percentages and numbers of GP33+ T cells in Il10f/f and Il10f/f Foxp3-Cre mice are shown. Data are from one experiment representative of 3 experiments (a, b, c, d) with 3-6 mice per group carried out 45-60 days (a, d), or 0, 8, and 15 days (b, c) following LCMV Armstrong infection (mean and s.e.m).

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