Supplementary Figure 4: Validation of RNA-seq results.

(a) Expression of Zeb2, Ccr7, Cx3cr1, and Pim1 was determined by qPCR analysis of cDNA isolated from pooled GP₃₃⁺ and NP₃₉₆⁺ CD8⁺ T cells at 15 days post acute LCMV infection from Il10^f/f and Il10^f/f Foxp3-Cre mice. Expression of TCF-1 and GzmB in GP₃₃⁺ in T cells at 15 days post acute LCMV infection from Il10^f/f and Il10^f/f Foxp3-Cre mice was determined by flow cytometric analysis. (b) Representative plots of the GP₃₃⁺ T cell response in Il10^f/f and Il10^f/f Foxp3^Cre mice at day 15 post LCMV infection. Data are from one experiment representative of 3 experiments with 3-5 mice per group carried out 15 days following LCMV Armstrong infection (mean and s.e.m).