Supplementary Figure 7: Transfer of IL-10-competent CD4⁺ T_reg cells during the resolution phase of LCMV infection is sufficient to ‘rescue’ the maturation defect of memory CD8⁺ T cells in Il10^–/– mice.

Analysis of the GP₃₃⁺ T cell response 60 days p.i. in Foxp3^GFP-DTR mice treated with diphtheria toxin at day -1, day 8, or day 15 p.i. or mock injected with PBS. (a) Percentage and numbers of GP₃₃⁺ T cells are shown. Representative of 3 independent experiments with 3-6 mice per group carried out 45-60 days following LCMV Armstrong infection. (b) Analysis of the GP₃₃⁺ T cell response 60 days post acute LCMV Armstrong infection in Il10^–/– mice and Il10^–/– mice that were administered 3x10⁵ Foxp3⁺ CD4⁺ T cells isolated from coinfected Foxp3^GFP-DTR mice at day 8 p.i. Percentage and numbers of GP₃₃⁺ T cells are shown. Representative of 2 independent experiments with 3-7 mice per group carried out 60 days following LCMV Armstrong infection. Data are from one experiment representative of 3 experiments (a) or 2 experiments (b) with 3-7 mice per group carried out 45-60 days following LCMV Armstrong infection (mean and s.e.m).