Supplementary Figure 1: IL-10 is required for optimal maturation of memory CD8⁺ NP396⁺ T cells.

(a) Analysis of the virus-specific CD8⁺ T cell response 60 days post acute LCMV Armstrong infection. The percentages and numbers of GP₃₃⁺ T cells along with the (b) representative plots and analysis of the NP₃₉₆⁺ T cell response in the spleen of wild type and Il10^–/– mice are shown. Percentages and numbers of NP₃₉₆⁺ T cells, along with the percentages of CD127⁺KLRG1^-, CD127^-KLRG1⁺, and CD62L⁺KLRG1^- cells in the NP₃₉₆⁺ T cell population are shown. (c) Mice were infected with acute LCMV and treated with αIL-10 between days 0-30, 0-8, 8-30, 15-30 or mock injected with PBS. Mice were sacrificed at day 30 and the percentage and numbers of GP₃₃⁺ T cells determined. Statistical analyses were performed using the unpaired two-tailed Student’s t-test. (*, p < 0.01; **, p < 0.001). Data are from one experiment representative of 5 experiments (a, b) or 3 experiments (c) with at least 4 mice per group carried out 45-60 days (a, b) or 30 days (c) following LCMV Armstrong infection (mean and s.e.m).