Supplementary Figure 2: IL-10 is required in a CD8+ T cell–extrinsic and CD8+ T cell–intrinsic manner to allow the maturation and survival of memory CD8+ T cells. | Nature Immunology

Supplementary Figure 2: IL-10 is required in a CD8+ T cell–extrinsic and CD8+ T cell–intrinsic manner to allow the maturation and survival of memory CD8+ T cells.

From: Production of IL-10 by CD4+ regulatory T cells during the resolution of infection promotes the maturation of memory CD8+ T cells

Supplementary Figure 2

50,000 Il10raf/f or Il10raf/fCd4-Cre P14 GP33+ cells were transferred into congenically-mismatched mice one day prior to infection with acute LCMV infection. Analysis of the P14 GP33+ T cell response was carried out 30 days p.i. (a) Representative plots and analysis of the P14 GP33+ T cell response. Percentages and numbers of P14 GP33+ T cells, along with the percentages of CD127+KLRG1-, CD127-KLRG1+, and CD62L+KLRG1- cells in the P14 GP33+ T cell population are shown. (b) Representative histograms of GzmB and Tcf1 expression in Il10raf/f (black) or Il10raf/fCd4-Cre (gray filled) P14 GP33+ cells. Statistical analyses were performed using the unpaired two-tailed Student’s t-test. (*, p < 0.05; **, p < 0.01). Data are from one experiment representative of 2 experiments with 4 mice per group carried out 30 days following LCMV Armstrong infection (mean and s.e.m).

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