Supplementary Figure 3: Peripheral T cells generated in a β5t-deficient thymus are abnormal.

(a) Frequencies of CD44^loCD122^lo and CD44^hiCD122^hi cells in CD8⁺ T cells from the spleens and lymph nodes of β5t-deficient Rag2-deficient OT-I-TCR-transgenic mice. (b) CD44 and CD122 expression in CD4⁻CD8⁺V_α2^hi mature OT-I-TCR-transgenic thymocytes. (c) Splenic CD8⁺ T cells in β5t-deficient mice at different ages were analyzed for the ratios of CD44^lo to CD44^hi cells. (d) Absolute numbers of naïve (CD44^loCD122^lo) and memory-like (CD44^hiCD122^hi) CD8⁺ T cells in polyclonal TCR-expressing β5t-deficient mice at different ages. (e) Thymocytes from β5t-deficient and control mice were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) and adoptively transferred to sublethally irradiated (6.0 Gy) B6-Ly5.1 mice (1×10⁶ SP thymocytes/recipient). CD45.2⁺ donor cells in recipient spleens were analyzed for CFSE dilution as well as CD4, CD8, and CD44 expression 14 days after transfer. (f) Representative histograms of CD8⁺ T cells bound to gp33–H-2D^b tetramers in the experiments shown in Fig. 3c. Accumulated results from more than six (a and b) or three (c and d) mice/group and representative data from two (e) or three (f) independent experiments are shown. Graphs show data of individual mice (circles) and means (bars) (a and b). Circles and bars indicate average ± standard errors of the mean (c-e). ***P < 0.001, **P < 0.01 *P < 0.05.