Supplementary Figure 2: Administration of IL-33 and TSLP induces TH2 cells to produce IL-13.
(a) Naïve CD4 T cells were sorted from OT-II–4C13R reporter mice and cultured under TH2 conditions for three rounds. Cells were rested in IL-7-containing medium for 10 days and then injected i.v. into wild-type C57BL/6 recipient mice. Twenty-four hours later, mice were intratracheally challenged with PBS or indicated cytokines (150 ng IL-33 and/or 150 ng TSLP each mouse) for 3 consecutive days, or OVA (100 μg endotoxin-free OVA in PBS) once. Lungs were collected and cytokine production was examined 24h after last cytokine administration. Cells shown were gated on transferred OT-II TH2 cells. (b) Statistical analysis of the cytokine production. Error bars represent standard deviation from the mean. ****, P<0.0001; ns, P>0.05 by two tailed student’s t-test. Data are representative of two independent experiments with 4 mice in each group.
