Supplementary Figure 2: Early common progenitor, myeloid, erythroid and T lymphocyte cell development is unaltered in Brwd1^mut (Mut) animals.

(a) Absolute numbers of cells per mouse at different stages of B cell development in the BM of WT and Brwd1^mut/WT heterozygous (Het) mice (n = 3). (b) Differential blood counts of WT and Brwd1^mut mice (n = 3). (c) Flow cytometry analysis of common myeloid progenitors (CMP; Lin⁻Sca1⁻cKit^hiFcγR^loCD34^hi), granulocyte and macrophage progenitors (GMP; Lin⁻Sca1⁻cKit^hiFcγR^hiCD34^hi) and megakaryocyte and erythroid progenitors (MEP; Lin⁻Sca1⁻cKit^hiFcγR^loCD34^lo) from BM of WT and Brwd1^mut mice (n = 3). (d) Cellularity of myeloid progenitor cells in the BM of WT and Brwd1^mut mice (n = 3). (e) Flow cytometric analysis of BM erythroid cells of WT and Brwd1^mut mice (n = 3). (f) Cellularity of erythroid cells at different developmental stages includes proerythroblasts (population I, Ter119^medCd71^hi), basophilic erythroblasts (population II, Ter119^hiCd71^hi), and late erythroblasts (population III–IV, Ter119^hiCd71^med, Ter119^hiCd71^lo) (n = 3). (g) Flow cytometric analysis of BM myeloid cells of WT and Brwd1^mut mice (n = 3). (h) Cellularity of Gr1⁺Mac1⁺ cells in the BM of WT and Brwd1^mut mice (n = 3). (i) Flow cytometric analysis identifying LSK (Lineage⁻Sca1⁺cKit⁺), HSC (hematopoietic stem cells), MPP (multipotent progenitors), LMPP (lymphoid restricted multipotent progenitors) and CLP (common lymphoid progenitors) in the BM of WT and Brwd1^mut mice (n = 3). (j) Total progenitor cell numbers of LSK, HSC, MPP, LMPP and CLP progenitors in BM of WT and Brwd1^mut mice (n = 3). (k) Flow cytometric analysis of thymocytes identifying SP (single-positive, CD4⁺ or CD8⁺), DP (double-positive, CD4⁺CD8⁺) and DN (double-negative, CD4⁻CD8⁻) populations in WT and Brwd1^mut mice. The DN population was further analyzed by CD25 and CD44 staining to identify DN1, DN2, DN3 and DN4 populations (right panel of j) (n = 4). (l) Absolute numbers of DNs, CD4 and CD8 populations in the thymus of WT and Brwd1^mut mice (n = 4). All data in bar graphs are presented as average ± s.d.