Supplementary Figure 4: H3K9Ac and H3S10pK14Ac histone marks do not require BRWD1.
From: Histone reader BRWD1 targets and restricts recombination to the Igk locus
(a) ChIP with IgG or BRWD1-specific antibodies in flow-sorted small pre-B cells isolated from WT and Brwd1mut (Mut) mice followed by quantitative PCR for Jκ1–Jκ2 and Jκ4–Jκ5 regions. Background IgG subtracted from each lane. (b,c) ChIP with IgG, H3K9Ac (b) and H3S10pK14Ac (c) specific antibodies in flow-sorted pro-B and small pre-B cells isolated from WT and Brwd1mut mice followed by quantitative PCR with non-overlapping primer sets (Supplementary Table 1) designed to detect various regions of Igk. Data are representative of three independent experiments (average ± s.d.).
