Supplementary Figure 5: De novo motifs in BRWD1, H3K9Ac and H3S10pK14Ac ChIP-Seq peaks.
From: Histone reader BRWD1 targets and restricts recombination to the Igk locus
(a) Representative BRWD1, H3K9Ac and H3S10pK14Ac ChIP-Seq alignment (chromosome 1 and chromosome 6). A region (40,000K–110,000K) is zoomed in on (middle panel). Data are representative of two independent experiments. (b) Percentage of ChIP-Seq peaks (P < 10−7) alone or in combination in different genomic regions. Promoter (–5 kb to +500 bp from TSS); Intragenic (in exons or introns); Intergenic (not in promoter, exons and introns). (c) ChIP-qPCR with IgG and BRWD1-specific antibodies in flow-sorted immature B cells from Igkdel animals and for indicated regions of Igl (expressing immunoglobulin λ-chain). Data are representative of two independent experiments (average ± s.d.). (d) De novo DNA sequence motifs identified among BRWD1, H3K9Ac and H3S10pk14Ac-bound regions. (e,f) De novo DNA sequence motifs identified in BRWD1/H3S10pK14Ac (d) and BRWD1/H3K9Ac/H3S10pK14Ac (e) binding regions.
