Supplementary Figure 1: Brwd1 transcripts in Brwd1^mut B cells.

(a) Analysis of Brwd1 transcripts in isolated small pre-B (Lin⁻B220⁺CD43⁻IgM⁻FSC^lo) and splenic B (B220⁺) cells from WT and Brwd1^mut bone marrow (BM) and spleen by PCR using cDNAs prepared from those cells with several Brwd1 primer sets (Supplementary Table 1) that amplify different regions of Brwd1 transcript. (b) DNA sequence chromatograms from homozygous WT and Brwd1^mut animals across the exon 10–intron 10 junction, as indicated. There is a T-to-C change in the Brwd1^mut allele (n = 10). (c) Sequencing of aberrant PCR products obtained with primer set 2 (Supplementary Table 1) that amplifies exon 9–11 of cDNA prepared from isolated small pre-B cells of WT and Brwd1^mut BM with predicted translated amino acid sequences (n = 5). (d) Immunoblot analysis of BRWD1 on total nuclear cell lysates from splenic B cells isolated from WT and Brwd1^mut mice (n = 3).