Supplementary Figure 1: Loss of Mst1 and Mst2 increases susceptibility to bacterial sepsis.

(a) H&E staining of colon and kidney sections from wild type and Mst1^-/-Mst2^fl/flVav-Cre mice. Scale bar, 50 μm.

(b, c) Representative spleen tissues: spleen weight (b) and H&E staining of spleen sections (c) from wild type and Mst1^-/-Mst2^fl/fl Vav-Cre mice. Scale bar, 100 μm.

(d) White blood cell (WBC), circulating lymphocyte (LYM), monocyte (MON) and granulocyte (GRA) counts in Mst1^fl/flMst2^fl/fl (WT) and Mst1^fl/flMst2^fl/flLyz2-Cre (cDKO) mice. Each dot represents an individual mouse, and horizontal bars indicate the mean.

(e) Flow cytometric analysis of Gr-1⁺CD11b⁺ neutrophil and F4/80⁺CD11b⁺ macrophage populations in the bone marrow and spleens of WT and cDKO mice, as determined with anti-Gr-1, anti-F4/80 and anti-CD11b antibodies.

(f) Flow cytometric analysis of B220⁺ B cell, CD3⁺ T cell, the naïve T cell (CD62L^highCD44^low) and the effector T cell (CD62L^lowCD44^high) populations in the spleen, lymph node (LN) or blood of WT and cDKO mice, with the indicated antibodies.

(g) Flow cytometric analysis of Gr-1⁺CD11b⁺ neutrophil and F4/80⁺CD11b⁺ macrophage populations in blood of WT and cDKO mice subjected to cecal ligation and puncture (CLP) for 24h.

(h) H&E staining of kidney sections from WT or cDKO mice subjected to sublethal CLP. Scale bar, 50 μm.

(i) WT BMDMs were stimulated with different TLR agonists for the indicated times, followed by immunoblot analysis with the indicated antibodies.

Data were assessed with Student’s t-test and are represented as mean ± s.d. ns, not significant, * p<0.05, *** p<0.001 compared with respective controls or as indicated (b, n=10; d, n=8; e, n=3; g, n=4). Data are representative of three independent experiments.