Supplementary Figure 6: MHC class II expression in LCs is required for the generation of T_reg cells after IR treatment.

(a,b) Foxp3-DTR mice were exposed to 6 Gy IR followed by the subcutaneous inoculation of 5 x 10⁵ B16 tumor cells 12 h later. 5 d after tumor challenge, and every 2 d thereafter, mice were inoculated with DT i.p. (a) Tumor volumes were assessed at the indicated time points. (b) Tumor infiltrating and tdLN Foxp3⁺ T_reg cells were assessed 12 d after tumor challenge by flow cytometry. Shown is the mean +/− SD of 3 animals in one experiment. (c, d) H2^−/− mice were lethally irradiated and reconstituted with BM cells isolated from either WT or Cdkn1a^−/− mice. Two months post-transplantation, mice were further 6 Gy IR-treated and injected subcutaneously with 5 x 10⁵ B16 melanoma cells 12 h later. (c) Graph depicting tumor volumes as the mean +/− SD of n=4 mice in two different experiments. (d) Tumor infiltrating T_reg cells are shown as the percentage of total hematopoietic cells (gated as CD45⁺ cells) in B16 tumors at 14 d post-B16 injection. Data are pooled from two independent experiments, n≥2 mice per experiment. Data are plotted as Mean +/− SD. p values of ≤0.01 are labeled as ** and p≤0.001 as ***.