Supplementary Figure 9: TCR-CD3 surface expression on SKW3-FS18 cell lines
From: T cell receptor reversed polarity recognition of a self-antigen major histocompatibility complex
1x105 SKW3.FS18 T cells, wt and mutants, were stained with V450-conjugated mouse anti-human CD3 (UCHT1; BD Pharmingen) and analyzed by flow cytometry. MFI of CD3-V450 was calculated in FlowJo and plotted in Prism (GraphPad Software). Quantified results are the means ± SD of four replicates and representative of three independent experiments.
