Supplementary Figure 2: Phenotypic analyses of iTreg clones
From: T cell receptor reversed polarity recognition of a self-antigen major histocompatibility complex
(A) Mean fluorescence intensity expression of surface markers by iTreg clones (green) compared to effector T cell clones (red). Green circle shows a representative staining for iTreg clone FS18. Box and whiskers were generated according to Tukey. ***p<0.001, **p<0.01; 2-way ANOVA (for CD4, αβTCR, CD28 and GITR) and Mann-Whitney test (for CD62L). (B) Proliferation of clones derived from the Treg line upon stimulation with pro-insulin-loaded PBMC (black bars) or CMV-loaded PBMC (white bars). (C) IL-10 production by iTreg clones FS25 and FS17 upon stimulation with PBMC loaded with different concentrations of pro-insulin peptide.
