Supplementary Figure 1: RNA-Seq analyses of HSC and lymphoid populations from human BM and thymus. | Nature Immunology

Supplementary Figure 1: RNA-Seq analyses of HSC and lymphoid populations from human BM and thymus.

From: Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages

Supplementary Figure 1

Fluorescence activated cell sorting was used to isolate populations from (a) CD34+ enriched bone marrow cells (events pre-gated on DAPI-Lin- cells are depicted, * lineage cocktail included an antibody to CD19 except in the case of the BCP population), and (b) thymic cells; either CD34+ enriched (events pre-gated on CD34+CD4-CD8- cells are depicted) or CD34neg cells (events pre-gated on DAPI- cells are depicted). (c) RNA-Seq expression data of protein coding genes previously described to be associated with HSC or lymphoid lineages (n=2 biological replicates shown for each population). (d) Coding potential of transcripts from the entire dataset, estimated using CPAT and PhyloCSF algorithms. Novel lncRNAs include the set of transcripts just prior to selection of predictions with low coding potential (Fig. 1b). Intergenic lncRNAs: transcripts with no genomic overlap with protein coding genes. Divergent lncRNAs: transcripts from lncRNA genes that have antisense overlap with a protein coding gene. Data for transcripts from protein coding and annotated lncRNA databases are depicted for comparison.

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