Supplementary Figure 7: IFN-γ-mediated inhibition of the proliferation and cytokine production of ILC2 cells occurs independently of STAT2, IRF9, IFNAR, STAT4, T-bet, iNOS and STAT6. | Nature Immunology

Supplementary Figure 7: IFN-γ-mediated inhibition of the proliferation and cytokine production of ILC2 cells occurs independently of STAT2, IRF9, IFNAR, STAT4, T-bet, iNOS and STAT6.

From: Type I interferon restricts type 2 immunopathology through the regulation of group 2 innate lymphoid cells

Supplementary Figure 7

Bone marrow cells from (a) WT and Stat2-/-, (b) WT and Irf9-/-, (c) WT and Ifnar1-/-, (d) WT and Stat4-mutant, (e) WT and Tbx21-/-, (f) WT, iNos-/- and Stat6-/- mice were stimulated for five days in medium as a control or with IL‐7 and IL-33 (both at 10 ng/ml). IFN-γ (5 or 20 ng/ml) was added to some wells, as indicated. Proliferation of cells was measured by alamarBlue assay, cytokine production was determined by ELISA of cell culture supernatants. The bars represent the mean ± standard deviation of triplicates of each cohort. Data are representative of three independent experiments. RFU, relative fluorescence units; n.d., not detectable; ns, not statistically significant (p > 0.05). The asterisks indicate statistically significant differences (*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001).

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