Supplementary Figure 4: Dose-dependent suppression of ILC2 function by IFN-γ and IL-27 in vitro. | Nature Immunology

Supplementary Figure 4: Dose-dependent suppression of ILC2 function by IFN-γ and IL-27 in vitro.

From: Interferon and IL-27 antagonize the function of group 2 innate lymphoid cells and type 2 innate immune responses

Supplementary Figure 4

ILC2 cells were isolated from mesentery, cultured with 10 ng/ml IL-2 and then seeded (5,000 cells/well) with indicated doses of IFN-γ (10 ng/ml) and IL-27 (10 ng/ml) in the presence of 10 ng/ml IL-33. ILC2 cells were counted by flow cytometry and amounts of IL-5 and IL-13 in the supernatants were measured by ELISA on day 6.

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