Supplementary Figure 8: Gating strategy for ILC2 cells in flow cytometry. | Nature Immunology

Supplementary Figure 8: Gating strategy for ILC2 cells in flow cytometry.

From: Interferon and IL-27 antagonize the function of group 2 innate lymphoid cells and type 2 innate immune responses

Supplementary Figure 8

BALF cells and lung cells were isolated from C57BL/6 mouse 5 days after intratracheal injection of 0.5 μg/mouse IL-33 on days 0 and 2. Mesentery cells (FALC) were isolated from untreated C57BL/6 mouse. Cells were stained with indicated antibodies and examined by flow cytometry. FSC-Alo SSC-Alo gates were used for the detection of ILC2 cells, and FCS-AloSSC-Ahi gates were used for the detection of eosinophils. FSC-H, FSC-W, SSC-H and SSC-W were used to gate out doublet cells. Note that FSCloSSChiSiglec-Fhi cells are all eosinophils.

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