Supplementary Figure 4: Effects of tumor and glucose on T cell function and tumor inhibitory B7 expression. | Nature Immunology

Supplementary Figure 4: Effects of tumor and glucose on T cell function and tumor inhibitory B7 expression.

From: Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction

Supplementary Figure 4

(a,b) Effects of tumor and glucose on polyfunctionality of CD4+ T cells. CD4+ T cells were activated with anti-CD3 and anti-CD28 for 3 days in normal medium, tumor medium, or these media supplemented with glucose. Polyfunctional profile was assessed by FACS. Results are shown as the mean of double (a) and triple (b) positive cells ± SEM of 3 donors. Mann-Whitney test, *P < 0.05, compared to medium.

(c) Effect of different concentrations of glucose on the total numbers of double- and triple-positive cells. CD8+ T cells were activated with anti-CD3 and anti-CD28 in different concentrations of glucose. The cells were analyzed by flow cytometry after 3 days. Data are shown as the mean ± SD, N = 4 donors. *P < 0.05.

(d) Effect of glucose restriction on T cell apoptosis. CD8+ T cells were stimulated for 3 days with anti-CD3 and anti-CD28 antibodies. Apoptosis was analyzed by Annexin V stanining. Data are presented as the mean ± SD, N = 4 donors, Wilcoxon rak-sum test, *P < 0.05.

(e) Effect of glucose on the expression of tumor B7H1 and B7H4. Ovarian tumor cells were cultured for 24 hours in the presence or absence of glucose. B7H1 and B7H4 expression was determined by FACS. One of two independent experiments is shown.

(f) Effect of 2-DG on viability of CD8+ T cells. CD8+ T cells were activated with anti-CD3 and anti-CD28 for 3 days in the presence or absence of 2-DG. Data are shown as the mean ± SEM of 4 donors. Wilcoxon rak-sum test, *P < 0.05.

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