Supplementary Figure 6: Relationship between EZH2, microRNAs and T cell function. | Nature Immunology

Supplementary Figure 6: Relationship between EZH2, microRNAs and T cell function.

From: Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction

Supplementary Figure 6

(a) Effects of microRNA mimics on EZH2 expression. Fresh blood CD8+ T cells were transfected with empty plasmid (control band) or plasmid encoding EZH2 overexpression, then treated with microRNAs mimics. After 24 hours, T cells were harvested and EZH2 was analyzed by Western blotting. Representative data, one of 3 donors tested.

(b-d) Effects of microRNA mimics on Bcl2 (b) and Hey1 (c) expression and polyfunctional CD8+ T cells (d). Fresh blood CD8+ T cells were transfected with control plasmid or plasmid encoding EZH2 overexpression, and subsequently co-transfected with microRNA mimics. The cells were activated with anti-CD3 and anti-CD28 after 3 days. Bcl2 and Hey1 expression was determined by real-time PCR. Polyfunctional CD8+ T cells were analyzed by FACS. Data presented as mean ± SEM, N=5 donors, Wilcoxon rank-sum test, *P < 0.05.

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