Supplementary Figure 2: Effects of DZNep on pro-apoptotic and anti-apoptotic gene expression. | Nature Immunology

Supplementary Figure 2: Effects of DZNep on pro-apoptotic and anti-apoptotic gene expression.

From: Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction

Supplementary Figure 2

(a) Kinetic expression of EZH2 in T cells. T cells were stimulated with anti-CD3 and anti-CD28 antibodies for the indicated time. EZH2 protein was detected with Western blotting. One representative experiment of 6 is shown.

(b,c) Effects of DZNep on Bcl-2 and pro-apoptotic gene expression. T cells were activated in the presence of 5 µM DZNep for 2 days. Expression of Bcl-2 (b), Bak, Bax and BIM (c) was quantified by real-time PCR. Results are shown as the relative expression to control (mean ± SEM). N = 5, Wilcoxon rank-sum test, *P < 0.05.

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