Supplementary Figure 3: Effect of Notch signaling inhibitor on T cell viability and Bcl-2 promoter activity. | Nature Immunology

Supplementary Figure 3: Effect of Notch signaling inhibitor on T cell viability and Bcl-2 promoter activity.

From: Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction

Supplementary Figure 3

(a) Effect of Notch signaling inhibitors on cell viability. CD8+ T cells were activated with anti-CD3 and anti-CD28 in the presence of the γ-secretase inhibitors, DAPT or GSI-I. The cells were harvested after 3 days and counted on a hemocytometer using Trypan blue exclusion of dead cells. Data presented as mean ± SEM. N = 4, Mann-Whitney U testet, *P < 0.05.

(b) Effects of Notch signaling on Bcl-2 promoter activity. 293T cells were co-transfected with hBCL-2-EGFP vector, control vector, and vectors encoding Notch dominant negative (Notch-DN) or Notch intracellular domain (Notch-IC) for 2 days. The promoter activity was analyzed by FACS and expressed as the percentage of GFP expression (mean ± SD). N = 4, Mann-Whitney U test, *P < 0.05.

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