Supplementary Figure 6: Functional Foxp3 is required for _ICT_N cells to regulate antigen-specific responses.

(a,b) Mean time of tumor onset for data described in (main Fig. 6a,b), respectively. Incidence of tumor-bearing mice as a fraction of the total mice in each group is shown below. (c) RIP-mOva/RAG^KO host mice received pIC-primed sf DR cells (sf DR, Donor pIC), or received ctr sf DR cells (sf DR, no tx), and blood glucose was monitored. Graph shows diabetes-free survival. P-value by one-tailed Mann-Whitney U-test, p<0.05. (d) Experimental setup for data described in Fig. 6d-e. Donor DR mice were treated with pIC or PBS as described previously, then CD4⁺ T cells were isolated and transferred into Rip-mOva/RAG^KO host mice. One day after transfer, all host mice were treated with pIC, and assessments conducted as described on day 20.