Supplementary Figure 5: Trabid regulates LPS-stimulated c-Rel recruitment and histone modifications at the promoters of Il12a and Il12b.

(a) ChIP assays of c-Rel recruitment to the promoter of the indicated genes using wild-type (WT) and DC-cKO BMDCs that were either unstimulated (0 h) or stimulated for 6 h with LPS. Data are presented as percentage of the total input DNA, as determined by QPCR assays. (b) Luciferase assays using HEK293 cells transfected with an Il12b-luciferase reporter plasmid in the presence (+) or absence (–) of an empty vector (Vector) or expression vectors encoding c-Rel, Trabid, or a catalytically inactive Trabid mutant, C443 (Mut Trabid). Luciferase activity is presented as fold based on empty vector. (c) ChIP analyses of H3K9 dimethylation (H3K9me2) and trimethylation (H3K9me3) in the Il12a promoter using wild-type or DC-cKO BMDCs that were untreated (NT) or stimulated with LPS for 6 h. The Y axis is percentage (%) of total H3. Data are presented as mean ± SEM values and representative of at least three independent experiments. Statistical analyses represent variations in technical replicates *P < 0.05; **P < 0.01.