Supplementary Figure 1: Zranb1 gene targeting. | Nature Immunology

Supplementary Figure 1: Zranb1 gene targeting.

From: Epigenetic regulation of the expression of Il12 and Il23 and autoimmune inflammation by the deubiquitinase Trabid

Supplementary Figure 1

(a) Schematic picture of Zranb1 gene targeting using an FRT-LoxP vector, showing the first 6 exons of Zranb1 gene (exons 7-9 are not shown). Targeted mice were crossed with FRT deleter (Rosa26-FLPe) mice to generate Zranb1-floxed mice, which were further crossed with different Cre mice to generate germline KO (CMV-Cre), T-cKO (Cd4-Cre), or DC-cKO (Cd11c-Cre) mice. (b) Genotyping PCR analysis of germline Zranb1 wild-type (WT), KO (KO), and heterozygous (Het) mice using P1/P2 primer pair for wild-type allele and P1/P4 primer pair for KO allele. (c,d) Genotyping PCR of T cell-conditional Zranb1 wild-type (WT, Zranb1+/+Cd4-Cre), T-cKO (Zranb1f/fCd4-Cre), and heterozygous (T-cKO Het, Zranb1+/fCd4-Cre) mice (c) or DC-conditional Zranb1 wild-type (WT, Zranb1+/+Cd11c-Cre), DC-cKO (Zranb1f/fCd11c-Cre), and heterozygous (DC-cKO Het, Zranb1+/fCd11c-Cre) mice (d) using P3/P4 primer pair to amplify WT and floxed alleles and Cre-specific primers for the Cd4-Cre and Cd11c-Cre DNA. (e) RT-PCR was performed to measure the Zranb1 mRNA level in the indicated cell types.

Back to article page