Supplementary Figure 2: Development of cells of the immune system in mice with germline deletion of Zranb1.

(a) Flow cytometry analysis of the percentage (numbers in quadrants) of CD4⁺CD8⁺ double-positive (DP), CD4⁺ and CD8⁺ single-positive (SP) thymocytes in the thymus (Thy) and B220⁺ B cells (B) and CD4⁺ and CD8⁺ T cells in the spleen (Spl) of wild-type (WT) and KO mice. (b) Flow cytometry analysis of naïve (N, CD62^hiCD44^lo) and memory (M, CD62^loCD44^hii) CD4⁺ T cells and naïve (N), central memory (CM, CD62L^hiCD44^hi), and effector memory (EM, CD62^loCD44^hi) CD8⁺ T cells in the spleen of wild-type and KO mice. (c) Flow cytometry analysis of the Foxp3⁺ Treg cells in the spleen and thymus of wild-type (WT) or KO mice. (d) Flow cytometry analysis of conventional DC (cDC, CD11c⁺CD11b^hiB220⁻) and plasmacytoid DC (pDC, CD11c⁺CD11b⁻B220⁺) percentage in total CD11c-positive cells of Bone marrow (BM) and Spleen (Spl). Data in all panels are presented as a representative FACS plots (left) and mean ± SEM values based on multiple samples (right). Similar results were obtained in three independent experiments. *P<0.05.