Supplementary Figure 2: CCP5 and CCP6 are involved in the innate immune response to DNA viruses.

(a) WT, Ccp5^−/− and Ccp6^−/− BMDMs were transfected with the indicated types of DNA or RNA (1 μg/ml) for 18 h, followed by IFN-β determination (upper panel) and IRF3 dimerization examination (lower panel). (b) Ccp6^−/− BMDMs rescued with CCP6-wt or CCP6-mut were incubated with HSV (MOI=1) for 8 h, followed by IRF3 dimerization examination. (c) Ccp6^+/+ and Ccp6^−/− BMDMs rescued with the indicated plasmids were infected with HSV-GFP (MOI=1) for 24 h, followed by microscopy examination. Scale bar, 400 μm. (d) WT BMDMs overexpressed with CCP5 or CCP6 for 24 h were infected with HSV-GFP (MOI=1) for 24 h, followed by microscopy examination (left panel). GFP positive cells were calculated (right panel). Scale bar, 400 μm. (e, f) WT, Ccp5^−/− and Ccp6^−/− BMDMs treated with 10 μM CoCl₂ (e) or 2 μM phenanthroline (f) for 6 h were infected with HSV-GFP (MOI=1) for 24 h, followed by microscopy examination. Scale bar, 400 μm. (g) WT, Ccp5^−/− and Ccp6^−/− BMDMs treated with 10 μM CoCl₂ or 2 μM phenanthroline for 6 h were incubated with VSV (MOI=1) for 8 h, followed by RT-PCR analysis of Ifnb. Data are shown as means±SD. ∗, P<0.05; **, P<0.01; ***, P<0.001. Data are representative of at least three independent experiments.