Supplementary Figure 4: Autophagy-dependent transcriptional profiles in Treg cells are responsive to rapamycin. | Nature Immunology

Supplementary Figure 4: Autophagy-dependent transcriptional profiles in Treg cells are responsive to rapamycin.

From: Autophagy enforces functional integrity of regulatory T cells by coupling environmental cues and metabolic homeostasis

Supplementary Figure 4

Foxp3CreAtg7+/fl and Foxp3CreAtg7fl/fl mice were treated with or without rapamycin (n=4 mice per group). Treg cells were sorted and activated with anti-CD3 and anti-CD28 for 4 h for gene expression profiling. (a) The top 10 canonical pathways upregulated in Foxp3CreAtg7fl/fl Treg cells as compared to Foxp3CreAtg7+/fl Treg cells by ingenuity pathway analysis (IPA) of the differentially expressed genes at the 0.5 log2 cut-offs. (b) Upstream regulators (kinases and transcription regulators) in Foxp3CreAtg7fl/fl Treg cells analyzed by IPA. Positive and negative activation z-scores (at the ± 2 cut-offs) indicate the activation and suppression of gene function in Foxp3CreAtg7fl/fl Treg cells respectively. (c,d) Heat maps of the expression changes of top hit genes in caspase (c) and cytokine pathways (d) in non-treated Foxp3CreAtg7fl/fl versus Foxp3CreAtg7+/fl Treg cells and rapamycin-treated Foxp3CreAtg7fl/fl versus Foxp3CreAtg7+/fl Treg cells. Red color denotes upregulated genes in Foxp3CreAtg7fl/fl Treg cells, and blue color denotes downregulated genes in Foxp3CreAtg7fl/fl Treg cells. Data are from one experiment (a-d).

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