Supplementary Figure 6: Autophagy regulates c-Myc expression in an mTORC1-dependent manner. | Nature Immunology

Supplementary Figure 6: Autophagy regulates c-Myc expression in an mTORC1-dependent manner.

From: Autophagy enforces functional integrity of regulatory T cells by coupling environmental cues and metabolic homeostasis

Supplementary Figure 6

(a) Expression of c-Myc in Treg cells (from Cd4CreAtg7+/flRptor+/fl, Cd4CreAtg7fl/flRptor+/fl, Cd4CreAtg7+/flRptorfl/fl and Cd4CreAtg7fl/flRptorfl/fl mice) stimulated with anti-CD3 and anti-CD28 for 4 h. Numbers above bracketed lines indicate percent c-Myc+ cells. (b) Flow cytometry analyzing YFP-Foxp3 expression in divided Treg cells (from Foxp3CreAtg7+/fl and Foxp3CreAtg7fl/fl mice) that were activated in vitro with anti-CD3 and anti-CD28, and IL-2 for 96 h in the presence of DMSO or i-BET-762. Numbers above graphs indicate MFI of YFP-Foxp3. Data are representative of three independent experiments (a ,b).

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