Supplementary Figure 6: The EAE phenotype of Cd3e−/−Il1b−/− chimera mice.
From: T cell–intrinsic ASC critically promotes TH17-mediated experimental autoimmune encephalomyelitis
(a) Lethal irradiated WT mice were reconstituted with WT+Cd3e-/- bone marrow or Il1b-/-+Cd3e-/- bone marrow. 6 weeks after reconstitution, mice were immunized with MOG35-55 peptide and 200 ng pertussis toxin on days 1 and 4. Graph represents the average clinical score after active immunization. (b) Inflammatory gene expression in the lumbar spinal cord was assessed at the peak of disease. (c) Absolute numbers of CNS-infiltrating cells were determined at the peak of disease. Brains and spinal cords were harvested together and mononuclear infiltrating cells were stained with anti-CD45, anti-CD4, anti-CD8, anti-F4/80, anti-Ly6C and anti-Ly6G antibodies, followed by flow cytometric analysis. Two-way ANOVA (a) and Unpaired t test (b and c) were used to analyzed the data. Data are representative of three independent experiments (a-c). n=5 mice per group in each experiment. Error bars represent s.e.m. *P < 0.05.
