Supplementary Figure 1: Generation of the Pycard conditional knockout allele.
From: T cell–intrinsic ASC critically promotes TH17-mediated experimental autoimmune encephalomyelitis
(a) The Pycard gene is localized on chromosome 7 and contains three coding exons. For generation of the conditional knockout allele, the entire open reading frame including all three exons was targeted for conditional deletion. After generation of the targeting vector (using Pcr4.0 backbone), C57BL/6 embryonic stem cells were injected for gene targeting. Southern hybridization was used to identify progeny bearing the recombinant allele. (b) CD4+ T Cells were sorted from Ascf/+Lck-Cre and Ascf/-Lck-Cre mice by flow cytometry, and mRNA expression of three different exons of ASC was measured by Real-time PCR analysis. Unpaired t test was used to analyze the data. Data are representative of three independent experiments (b). n=3 mice per group in each experiment. Error bars represent s.e.m. *P < 0.05.
