Supplementary Figure 4: Foxp3 inhibits the expression of SMS1.
From: Phosphatase PP2A is requisite for the function of regulatory T cells
(a) Jurkat T cells were spin-infected with a Thy1.1 expressing retrovirus that harbored or not the human FOXP3 coding sequence. Thy1.1+ cells were FACS-sorted 24 hours after infection and analyzed for FOXP3 and SGMS1 mRNA levels by real time PCR. (b) Immunoblot of protein extracts from control (GFP) or FOXP3 infected Jurkat T cells were probed for FOXP3 and SGMS1. Immunoblot (left) and relative quantification (right) of two distinct GFP-FOXP3 replicate pairs are shown. Results (a-b) are from one experiment representative of three independent experiments.
