Supplementary Figure 5: Activated PP2A interacts with the mTOR pathway in Treg cells.
From: Phosphatase PP2A is requisite for the function of regulatory T cells
(a) Jurkat T cells were treated with SMase (0.5 units/mL) or vehicle (50% glycerol in PBS) for 1h and then lysed for protein immunoprecipitation with a PP2AC-specific antibody. The indicated bands (arrows) were subjected to mass spectrometry. (b) The Ingenuity Pathway Analysis (IPA) of 269 proteins identified in (a) is shown. (c) Jurkat T cells treated as in (a) were lysed for protein immunoprecipitation with a PP2AC-specific antibody (upper) or a Raptor-specific antibody (lower). The immunoblots were then probed for PP2AC and Raptor. Image is from one experiment representative of two independent experiments with similar results.
