Supplementary Figure 7: Rapamycin normalizes the proportion of effector lymphocytes in PP2A^flox mice.

(a) The proportion of effector memory (CD44⁺CD62L^-) CD4⁺ T cells in blood was assessed before rapamycin treatment and in a weekly basis after treatment initiation. Progression in the percentage of cells is depicted (two-way ANOVA followed by Bonferroni’s test). (b) Numbers of effector memory CD4⁺ and CD8⁺ T cells in spleen from rapamycin-treated and untreated PP2A^wt and PP2A^flox mice after 4 weeks of treatment (ANOVA followed by Tukey’s multiple comparison test). (c, d) Percentage of GC (GL-7⁺FAS⁺) B cells (c) and T_fh cells (PD-1⁺CXCR5⁺) within CD4⁺ T cells (d) in spleens from (b) are shown. Cumulative (a and b) and representative (c and d) data from two experiments with similar results are demonstrated. Mean ± s.e.m. is shown, *P<0.05, **P<0.01, ***P<0.001.