Supplementary Figure 7: Non-centripetal motion of Zap70 clusters.
From: Initiation of T cell signaling by CD45 segregation at 'close contacts'
(a) Zap70 cluster trajectories (colour-coded with time). (b) Schematic depiction of the trajectory analysis: the vector from the center of the contact to the end of a given trajectory is calculated as well as the vector from the start to the end of the trajectory. The angle between the two vectors is 0/180° for centripetal motion, and takes a random value for random motion. (c) Histogram showing the frequencies for all calculated angles γ for Zap70 trajectories observed in Jurkat and JRT3-T3.5 cells during the first 180 s post contact formation with a rCD2 bilayer. Total number of trajectories analyzed: 2234 (Jurkat), 2158 (JRT3-T3.5), total number of analyzed cells: 28 (Jurkat), 36 (JRT3-T3.5). The data were collected in at least three independent experiments.
